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Extraction of extraradical arbuscular mycorrhizal mycelium from compartments filled with soil and glass beads

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Abstract

This study presents a novel method for the extraction and quantification of extraradical mycelium (ERM) of arbuscular mycorrhizal fungi (AMF) from a substrate that simulates soil better than previously used artificial growth media. Fungal compartments were constructed from small net pots with a latticed wall and filled with a mixture of glass beads and 40 μm wet sieved soil. The net pots were surrounded by a 30-μm mesh membrane through which hyphae but not roots could grow. They were inserted into soil where a Glomus intraradices (BEG 110) colonized potato plant was growing. The ERM that had grown out from roots through the membrane was successfully collected and quantified after harvest by washing out the soil/glass bead mixture through a sieve with a mesh width of 40 μm. Concentrations of P, Zn, Cu and Mn in the AMF ERM were analysed.

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Acknowledgements

This work was financed by the EU MYCHINTEC INCO-DEV Project no ICA4-CT-2000-30014. E.G. received an ARC IREX Fellowship (in cooperation with Prof. Zed Rengel) during the initial phase of this study.

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Authors and Affiliations

  1. Institute of Plant Nutrition (330), Hohenheim University, 70593, Stuttgart, Germany

    Elke Neumann

  2. Soil Science and Plant Nutrition, School of Earth and Geographical Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, Western Australia, 6009, Australia

    Eckhard George

  3. Institute of Vegetable and Ornamental Crops (IGZ), 14979, Grossbeeren, Germany

    Eckhard George

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  1. Elke Neumann
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  2. Eckhard George
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Correspondence to Elke Neumann or Eckhard George.

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Neumann, E., George, E. Extraction of extraradical arbuscular mycorrhizal mycelium from compartments filled with soil and glass beads. Mycorrhiza 15, 533–537 (2005). https://doi.org/10.1007/s00572-005-0361-6

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  • DOI: https://doi.org/10.1007/s00572-005-0361-6

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